Digital Holography Enables Quantitative Phase Evaluation during Cellular Microsurgery
Abstract
Digital holography which enables quantitative phase evaluation during cellular microsurgery has been reported. Laser scissors have become an important tool that allows cell biologists to alter cellular structures in order to study their structure-function relationship. Digital holographic microscopy can be performed in real time, and it allows one to determine dynamic changes in the optical thickness profile of a transparent object with sub-wavelength accuracy. Quantitative phase imaging by digital holographic microscopy provided high spatial as well as temporal resolution to dynamic changes in cell thickness. A quantitative phase laser micro-irradiation system was even capable of evaluating dynamic changes in the sub-cellular structure (nucleolus) of a cell.
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