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SNAC-tag for sequence-specific chemical protein cleavage.

  • Author(s): Dang, Bobo
  • Mravic, Marco
  • Hu, Hailin
  • Schmidt, Nathan
  • Mensa, Bruk
  • DeGrado, William F
  • et al.
Abstract

Site-specific protein cleavage is essential for many protein-production protocols and typically requires proteases. We report the development of a chemical protein-cleavage method that is achieved through the use of a sequence-specific nickel-assisted cleavage (SNAC)-tag. We demonstrate that the SNAC-tag can be inserted before both water-soluble and membrane proteins to achieve fusion protein cleavage under biocompatible conditions with efficiency comparable to that of enzymes, and that the method works even when enzymatic cleavages fail.

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