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Integrated design, execution, and analysis of arrayed and pooled CRISPR genome-editing experiments
- Canver, Matthew C;
- Haeussler, Maximilian;
- Bauer, Daniel E;
- Orkin, Stuart H;
- Sanjana, Neville E;
- Shalem, Ophir;
- Yuan, Guo-Cheng;
- Zhang, Feng;
- Concordet, Jean-Paul;
- Pinello, Luca
Published Web Location
https://doi.org/10.1038/nprot.2018.005Abstract
This protocol describes how to set up arrayed and pooled CRISPR genome-editing experiments. It describes the design of sgRNAs using CRISPOR, the wet-lab implementations, and analysis of the generated results by CRISPResso.
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