Skip to main content
eScholarship
Open Access Publications from the University of California

UC Riverside

UC Riverside Previously Published Works bannerUC Riverside

Analysis of endocrine disruption in Southern California coastal fish using an aquatic multispecies microarray.

  • Author(s): Baker, Michael E
  • Ruggeri, Barbara
  • Sprague, L James
  • Eckhardt-Ludka, Colleen
  • Lapira, Jennifer
  • Wick, Ivan
  • Soverchia, Laura
  • Ubaldi, Massimo
  • Polzonetti-Magni, Alberta Maria
  • Vidal-Dorsch, Doris
  • Bay, Steven
  • Gully, Joseph R
  • Reyes, Jesus A
  • Kelley, Kevin M
  • Schlenk, Daniel
  • Breen, Ellen C
  • Sásik, Roman
  • Hardiman, Gary
  • et al.

Published Web Location

https://doi.org/10.1289/ehp.11627
Abstract

Background

Endocrine disruptors include plasticizers, pesticides, detergents, and pharmaceuticals. Turbot and other flatfish are used to characterize the presence of chemicals in the marine environment. Unfortunately, there are relatively few genes of turbot and other flatfish in GenBank, which limits the use of molecular tools such as microarrays and quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) to study disruption of endocrine responses in sentinel fish captured by regulatory agencies.

Objectives

We fabricated a multigene cross-species microarray as a diagnostic tool to screen the effects of environmental chemicals in fish, for which there is minimal genomic information. The array included genes that are involved in the actions of adrenal and sex steroids, thyroid hormone, and xenobiotic responses. This microarray will provide a sensitive tool for screening for the presence of chemicals with adverse effects on endocrine responses in coastal fish species.

Methods

We used a custom multispecies microarray to study gene expression in wild hornyhead turbot (Pleuronichthys verticalis) collected from polluted and clean coastal waters and in laboratory male zebrafish (Danio rerio) after exposure to estradiol and 4-nonylphenol. We measured gene-specific expression in turbot liver by qRT-PCR and correlated it to microarray data.

Results

Microarray and qRT-PCR analyses of livers from turbot collected from polluted areas revealed altered gene expression profiles compared with those from nonaffected areas.

Conclusions

The agreement between the array data and qRT-PCR analyses validates this multispecies microarray. The microarray measurement of gene expression in zebrafish, which are phylogenetically distant from turbot, indicates that this multispecies microarray will be useful for measuring endocrine responses in other fish.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
Current View