UCSF

Objective

The objective of this study was to determine if clinical dynamic PET/CT imaging with ¹¹C-L-methyl-methionine (¹¹C-MET) in healthy older women can provide an estimate of tissue-level post-absorptive and post-prandial skeletal muscle protein synthesis that is consistent with the more traditional method of calculating fractional synthesis rate (FSR) of muscle protein synthesis from skeletal muscle biopsies obtained during an infusion of L-[ring ¹³C₆] phenylalanine (¹³C₆-Phe).

Methods

Healthy older women (73 ± 5 years) completed both dynamic PET/CT imaging with ¹¹C-MET and a stable isotope infusion of ¹³C₆-Phe with biopsies to measure the skeletal muscle protein synthetic response to 25 g of a whey protein supplement. Graphical estimation of the Patlak coefficient K_i from analysis of the dynamic PET/CT images was employed as a measure of incorporation of 11 C-MET in the mid-thigh muscle bundle.

Results

Post-prandial values [mean ± standard error of the mean (SEM)] were higher than post-absorptive values for both K_i (0.0095 ± 0.001 vs. 0.00785 ± 0.001 min^-1, p < 0.05) and FSR (0.083 ± 0.008 vs. 0.049 ± 0.006%/h, p < 0.001) in response to the whey protein supplement. The percent increase in K_i and FSR in response to the whey protein supplement was significantly correlated (r = 0.79, p = 0.015).

Conclusions

Dynamic PET/CT imaging with ¹¹C-MET provides an estimate of the post-prandial anabolic response that is consistent with a traditional, invasive stable isotope, and muscle biopsy approach. These results support the potential future use of ¹¹C-MET imaging as a non-invasive method for assessing conditions affecting skeletal muscle protein synthesis.