Lawrence Berkeley National Laboratory

The genomic revolution ushered in an era of discovery and characterization of enzymes from novel organisms that fueled engineering of microbes to produce commodity and high-value compounds. Over the past decade advances in synthetic biology tools in recent years contributed to significant progress in metabolic engineering efforts to produce both biofuels and bioproducts resulting in several such related items being brought to market. These successes represent a burgeoning bio-economy; however, significant resources and time are still necessary to progress a system from proof-of-concept to market. In order to fully realize this potential, methods that examine biological systems in a comprehensive, systematic and high-throughput manner are essential. Recent success in synthetic biology has coincided with the development of systems biology and analytical approaches that kept pace and scaled with technology development. Here, we review a selection of systems biology methods and their use in synthetic biology approaches for microbial biotechnology platforms.