Lawrence Berkeley National Laboratory

Advances in synthetic biology now offer spatiotemporal, conditional, and precise control of plant metabolism, enabling engineering of pathways that have resisted constitutive approaches. Plant cell wall biosynthesis and metabolite glycosylation are industrially valuable pathways that are also acutely sensitive to constitutive perturbation. We use these pathways as a lens to examine the potential impact of emerging programmable tools, including synthetic, tissue-specific, and inducible promoter design; RNA-, protein-, and pathway-level regulatory switches; and CRISPR-based systems. We then examine how these tools are overcoming barriers in cell wall engineering, production of glycosylated therapeutics, and emerging applications in signalling and herbicide tolerance. Together, these advances move plant metabolic engineering from static modification toward self-regulating, precise metabolic control, with complex glycosylation emerging as both a significant test and a key beneficiary of these advances.