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ANTIBODY-BASED FIBEROPTICS BIOSENSOR FOR THE CARCINOGEN BENZO(A)PYRENE.

  • Author(s): Vo-Dinh, T
  • Tromberg, BJ
  • Griffin, GD
  • Ambrose, KR
  • Sepaniak, MJ
  • Gardenhire, EM
  • et al.
Abstract

A new antibody-based fiberoptics biosensor was used to detect the important carcinogen benzo(a)pyrene (BaP). The fiberoptics sensor utilizes anti-BaP antibodies covalently bound to its tip. A helium cadmium laser was used as the excitation source to induce fluorescence from BaP conjugated to the bound anti-BaP antibodies. The fiberoptics device can detect 1 femtomole of BaP in a 5- mu L sample drop.

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