Two-photon-induced fluorescence from the phycoerythrin protein
- Author(s): Chen, Z
- Kaplan, DL
- Yang, K
- Kumar, J
- Marx, KA
- Tripathy, SK
- et al.
Published Web Locationhttps://doi.org/10.1364/AO.36.001655
Two-photon-induced fluorescence is observed from the photodynamic phycobiliprotein phycoerythrin. Temporal, spectral, and intensity-dependent properties of the two-photon-induced fluorescence emission from phycoerythrin excited by a 1-06-σmlaser beam are reported. The measured two-photon absorption cross section of phycoerythrin is an order of magnitude larger than that of Rhodamine 6G. The potential applications of phycobiliproteins for two-photon-induced fluorescence for microscopy of three-dimensional biological samples and three-dimensional optical memory are discussed. © 1997 Optical Society of America.
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