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Two-photon-induced fluorescence from the phycoerythrin protein.

  • Author(s): Chen, Z;
  • Kaplan, DL;
  • Yang, K;
  • Kumar, J;
  • Marx, KA;
  • Tripathy, SK
  • et al.
Abstract

Two-photon-induced fluorescence is observed from the photodynamic phycobiliprotein phycoerythrin. Temporal, spectral, and intensity-dependent properties of the two-photon-induced fluorescence emission from phycoerythrin excited by a 1.06-mum laser beam are reported. The measured two-photon absorption cross section of phycoerythrin is an order of magnitude larger than that of Rhodamine 6G. The potential applications of phycobiliproteins for two-photon-induced fluorescence for microscopy of three-dimensional biological samples and three-dimensional optical memory are discussed.

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