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Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex
- Robinson, Philip J;
- Trnka, Michael J;
- Bushnell, David A;
- Davis, Ralph E;
- Mattei, Pierre-Jean;
- Burlingame, Alma L;
- Kornberg, Roger D
Published Web Location
https://doi.org/10.1016/j.cell.2016.08.050Abstract
A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription.
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