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Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II

  • Author(s): Sierra, RG
  • Gati, C
  • Laksmono, H
  • Dao, EH
  • Gul, S
  • Fuller, F
  • Kern, J
  • Chatterjee, R
  • Ibrahim, M
  • Brewster, AS
  • Young, ID
  • Michels-Clark, T
  • Aquila, A
  • Liang, M
  • Hunter, MS
  • Koglin, JE
  • Boutet, S
  • Junco, EA
  • Hayes, B
  • Bogan, MJ
  • Hampton, CY
  • Puglisi, EV
  • Sauter, NK
  • Stan, CA
  • Zouni, A
  • Yano, J
  • Yachandra, VK
  • Soltis, SM
  • Puglisi, JD
  • DeMirci, H
  • et al.
Abstract

We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (<3-Å diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-Å structure).

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