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Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II
- Sierra, Raymond G;
- Gati, Cornelius;
- Laksmono, Hartawan;
- Dao, E Han;
- Gul, Sheraz;
- Fuller, Franklin;
- Kern, Jan;
- Chatterjee, Ruchira;
- Ibrahim, Mohamed;
- Brewster, Aaron S;
- Young, Iris D;
- Michels-Clark, Tara;
- Aquila, Andrew;
- Liang, Mengning;
- Hunter, Mark S;
- Koglin, Jason E;
- Boutet, Sébastien;
- Junco, Elia A;
- Hayes, Brandon;
- Bogan, Michael J;
- Hampton, Christina Y;
- Puglisi, Elisabetta V;
- Sauter, Nicholas K;
- Stan, Claudiu A;
- Zouni, Athina;
- Yano, Junko;
- Yachandra, Vittal K;
- Soltis, S Michael;
- Puglisi, Joseph D;
- DeMirci, Hasan
- et al.
Published Web Location
https://doi.org/10.1038/nmeth.3667Abstract
We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (<3-Å diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-Å structure).