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Open Access Publications from the University of California

Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II.

  • Author(s): Sierra, Raymond G
  • Gati, Cornelius
  • Laksmono, Hartawan
  • Dao, E Han
  • Gul, Sheraz
  • Fuller, Franklin
  • Kern, Jan
  • Chatterjee, Ruchira
  • Ibrahim, Mohamed
  • Brewster, Aaron S
  • Young, Iris D
  • Michels-Clark, Tara
  • Aquila, Andrew
  • Liang, Mengning
  • Hunter, Mark S
  • Koglin, Jason E
  • Boutet, Sébastien
  • Junco, Elia A
  • Hayes, Brandon
  • Bogan, Michael J
  • Hampton, Christina Y
  • Puglisi, Elisabetta V
  • Sauter, Nicholas K
  • Stan, Claudiu A
  • Zouni, Athina
  • Yano, Junko
  • Yachandra, Vittal K
  • Soltis, S Michael
  • Puglisi, Joseph D
  • DeMirci, Hasan
  • et al.

We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (<3-Å diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-Å structure).

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