Element-to-calcium (X/Ca) ratios within biogenic calcium carbonate minerals are used as proxies to reconstruct past seawater temperature and composition. This study focuses on examining Li/Ca, B/Ca, and Mg/Ca ratios in sea urchins cultured at different temperatures and pCO2 levels.
In situ secondary ion mass spectrometry (SIMS) analyses were conducted on two species of sea urchins cultured under controlled conditions and several species of paleo-echinoderms. A temperate species, Arbacia punctulata, was cultured at various pCO2 levels (400, 600, 900, 2850 ppmv) with temperature held constant at 25°C. A tropical species, Echinometra viridis, was cultured in variable pCO2 (400 and 1000 ppmv) and temperature (20°C and 30°C) conditions.
In the tropical species, a positive correlation was found between temperature and the analyzed (Li, B, Mg) element-to-calcium ratios of the spines, while variable responses to pCO2 were observed. In the temperate species, a variable response to increasing pCO2 was observed, with B/Ca having a similar trend to the calcification rate found in a previous study by Ries et al. (2009). Paleo-echinoderm samples varied in age from 518 to 53 Ma, and have a global distribution. The X/Ca ratios from paleo-echinoderms were used, along with the full range of observed partition coefficients (DcX/Ca=X/Cacarbonate/X/Cafluid, with X=Li, B, or Mg in this study) from the cultured sea urchins, to model element-to-calcium ratios of paleo-seawater.