- Main
A mosaic monoploid reference sequence for the highly complex genome of sugarcane.
- Author(s): Garsmeur, Olivier
- Droc, Gaetan
- Antonise, Rudie
- Grimwood, Jane
- Potier, Bernard
- Aitken, Karen
- Jenkins, Jerry
- Martin, Guillaume
- Charron, Carine
- Hervouet, Catherine
- Costet, Laurent
- Yahiaoui, Nabila
- Healey, Adam
- Sims, David
- Cherukuri, Yesesri
- Sreedasyam, Avinash
- Kilian, Andrzej
- Chan, Agnes
- Van Sluys, Marie-Anne
- Swaminathan, Kankshita
- Town, Christopher
- Bergès, Hélène
- Simmons, Blake
- Glaszmann, Jean Christophe
- van der Vossen, Edwin
- Henry, Robert
- Schmutz, Jeremy
- D'Hont, Angélique
- et al.
Published Web Location
https://doi.org/10.1038/s41467-018-05051-5Abstract
Sugarcane (Saccharum spp.) is a major crop for sugar and bioenergy production. Its highly polyploid, aneuploid, heterozygous, and interspecific genome poses major challenges for producing a reference sequence. We exploited colinearity with sorghum to produce a BAC-based monoploid genome sequence of sugarcane. A minimum tiling path of 4660 sugarcane BAC that best covers the gene-rich part of the sorghum genome was selected based on whole-genome profiling, sequenced, and assembled in a 382-Mb single tiling path of a high-quality sequence. A total of 25,316 protein-coding gene models are predicted, 17% of which display no colinearity with their sorghum orthologs. We show that the two species, S. officinarum and S. spontaneum, involved in modern cultivars differ by their transposable elements and by a few large chromosomal rearrangements, explaining their distinct genome size and distinct basic chromosome numbers while also suggesting that polyploidization arose in both lineages after their divergence.