International Journal of Comparative Psychology

This paper describes how to use tardigrades to demonstrate habituation. This experiment is designed for students with any level of experience or training in conditioning live organisms. In this experiment, tardigrades are desensitized to repeated physical touch. Tardigrades are placed under a microscope and poked with a probe until the strength of their response decreases to the point where there is no reaction for 10 consecutive trials. Once the habituation criteria are reached, a new stimulus is presented as a dishabituation control to ensure the subject responds appropriately to the new stimuli. Dishabituation is essential to show that the original response is still present even when a different stimulus is used to evoke that response. This experiment is easy to perform, does not require a lot of time or tools, and the effects are easily observed. We have added discussion questions and future research ideas to aid instructors in the classroom.